Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps

Table 1 Results of lethality/sterility test (F₃ cross)

First round of single-pair matings		Second round of single-pair matings
phenotype	number of insertions	phenotype	number of insertions
viable*	4908 (86.8%)	viable	250 (4.4%)
potentially lethal	589 (10.4%)	lethal	421 (7.4%)
potentially sterile	160 (2.8%)	sterile	8 (0.1%)
		not retested	70 (1.2%)

A total of 5,657 lines were tested for potential lethality or sterility by a first round of single-pair matings (left half of table; see Results and Methods for details). Those that matched the criteria (749) were retested by a second round of additional single-pair matings in order to eliminate false positives (right half of table). Only those lines that matched the definition in the second round were considered to be lethal or sterile. All percentages are calculated based on the original total of 5,657 lines.
*This number includes 236 lines that were considered potentially semi-lethal (see text for definition of semi-lethality). Because this was done only on a subset of 2,940 lines, the numbers are not given separately.

ISSN: 1741-7007