Table 1 Protein sequences of pectinolytic enzymes in ant fecal fluid and corresponding molecular weights in daltons
Protein | Mass spectrometry dataa | Sequence data | Bandb | MW (Da) |
|---|---|---|---|---|
Arabinofuranosidase | FPGGNNJENTVDQR | FPGGNNLENTVDQR | 4 | 69139 |
GD[YQ/QY]JPSTJPSSTGTVFWSVVR | GDQYIPSTLPSSTGTVFWSVVR | |||
NDJASAJAEVG[SP/PS]FWR | NDIASALAEVGPSFWR | |||
PEDFAANTYTFR | NEDFFAANTYTFR | |||
STAJPNAJHVVJPTGR | STALPNALHVVIPTGR | |||
VESAAGEAAFMTGJER | VESAAGEAAFMTGLER | |||
YFE[W/GE]YAAJSTNNP/PNDJFG | YFEGEYAAISTNPNDIFG | |||
Endogalactanase | DJDGJNTQJFTYTR | DLDGLNTQIFTYTR | 7 | 37416 |
GAVTPFEEJJHNHGA[J/N] | GAVTPFEELLHNHGAN | |||
GWFSSJANJESSGR | GADFSSLANLESSGR | |||
Pectin esterase | GQAYFGGNTJ[QR/GVK] | GQAYFGGNTLGVK | 6 + 7 + 8 + 9 + 10 + 18 + 19 + 22 | 37571 |
GAGWVTASGR | GAGWVTASGR | |||
GYJEGATDFJFGQR | GYIEGATDFIFGQR | |||
NNQATJQFGJDAGQAGSDDASGTJR | NNQATIQFGLDAGQAGSDDASGTLR | |||
NTFGVGSQAJAJSQYGDR | NTFGVGSQAIALSQYGDR | |||
QAYFGGNTJGVK | QAYFGGNTIGVK | |||
Pectate lyase | JVJJSGNJSGDAVVR | IVLLSGNLSGDAVVR | 7 + 8 | |
VJNENNVJJR | VLNENNVIIR | |||
Polygalacturonase | VAVN(1710)...(1206)TGTWNWSNJK | VAVNCGVGSCTGTWNWSNLK | 6 | 37055 |
Rhamnogalacturonan acetylesterase-1 | PDNJWVNGEJGAGPR | PDNIWVNGEIGAGPR | 10 + 16 + 17 + 18 + 19 | 26946 |
FVGYAQTAASR | FVGYAQTAASR | |||
VNDAJAGR | VNDAIAGR | |||
Rhamnogalacturonan acetylesterase-2 | FVTYAQSJGJR | FVTYAQSLGLR | 18 | 26467 |