Figure 9
From: ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function
Suppressor of cytokine signalling (SOCS) box-dependent ubiquitination of creatine kinase B (CKB) and ubiquitous mitochondrial CK (uMtCK) by human ankyrin repeat and SOCS box protein 9 (ASB9). Haemagglutinin (HA)-tagged uMtCK was transiently cotransfected with Flag-ubiquitin (Ub) into HEK293 cells that stably express null (empty vector), Myc-tagged ASB9 or ASB9ΔSOCS. The cells were pretreated with MG132 for 6 h prior to the cell harvest when indicated. Cell lysates (a) and the anti-Myc antibody immunocomplexes (b) were blotted with anti-Myc, anti-Flag, anti-HA or anti-CKB antibodies.