Figure 2From: A next-generation sequencing method for overcoming the multiple gene copy problem in polyploid phylogenetics, applied to Poa grassesProbability of observing all alleles for one nuclear region vs. number of sequence reads obtained. 10,000 draws with replacement were simulated for each sequence read number. Black lines show the case of equal allele proportions where the true allele number is 2 (dotted line), 3 (dashed line) and 4 (solid line). Red and blue dotted lines show the case for the observed copy proportions for CDO504 and DMC1 respectively. Grey vertical lines show the read number required to observe all alleles with >0.8 and >0.95 probability when 4 alleles are actually present.Back to article page