Figure 8From: A next-generation sequencing method for overcoming the multiple gene copy problem in polyploid phylogenetics, applied to Poa grassesDiagram of barcoded amplicon. The amplicon of interest, flanked by forward and reverse PCR primer sequences, was subjected to ligation of the Titanium Adapter B and Adapter A + barcode sequence, producing an oligonucleotide of the form shown. One out of 64 barcode + check-sum base sequences is represented here. Initially, the Adapter A oligonucleotide used was 6 bp shorter at the 5' end. It was extended by PCR to match the Titanium sequence shown here, as described in the text.Back to article page