Figure 2
From: Sumo-dependent substrate targeting of the SUMO protease Ulp1
Small ubiquitin-like modifier (SUMO) is required for the localization of Ulp1(C580S) to the septin ring. (A) The indicated mutants smt3-331, ubc9-1, smt3-R11, 15, 19, siz1Δ siz2Δ (YOK 1995, YOK 2065, YOK 1910 and YOK 2067) and a WT control strain (WT) were transformed with a plasmid expressing GFP-tagged Ulp1(C580S). Representative images indicating the localization of GFP-tagged Ulp1(C580S) after G2/M arrest are shown. The septin ring localization of Ulp1(C580S) is indicated where present (arrowheads). Note that Ulp1(C580S) failed to localize to the septin ring in SUMO-conjugating and ligating enzyme mutants (ubc9-1 and siz1Δ siz2Δ, respectively). (B) Septin ring localization of Smt3-GFP is absent in ubc9-1 and siz1Δ siz2Δ strains. Localization of Smt3-GFP was visualized in G2/M-arrested WT, ubc9-1 and siz1Δ siz2Δ strains (YOK 1857, YOK 2144 and YOK 2143) using fluorescence microscopy. Arrowheads indicate the position of the septin ring.