Figure 9
The effect of chicken CSF1-Fc treatment on bone density. Hatchlings were dosed subcutaneously with 50 μg chicken CSF1-Fc or PBS on hatch day (day 0), day 1, day 2 and day 3, then sacrificed 24 hours after the final injection. Femurs were fixed overnight in 4% paraformaldehyde, and then transferred to 70% ethanol. Micro-computed tomography analysis of the femur was carried out as described in Methods. Error bars represent standard error of the mean. Statistically significant differences were calculated using unpaired t-tests between treatment groups. PBS, phosphate-buffered saline.