Fig. 5

Irga6 co-localizes with lipid droplets in Irgm1/Irgm3 ^−/− cells. a Irgm1 ^−/−, Irgm3 ^−/−, and Irgm1/Irgm3 ^−/− mouse embryonic fibroblasts (MEFs) were induced with 200 U/mL IFN-γ for 24 hours. Cells were fixed and stained with anti-Irga6 antibody (10D7) and neutral lipid dye LD540. Representative microscopic images of Irga6 and lipid droplet co-localization are shown. Arrows point at the Irga6 structures magnified at the end of each panel in the following array: upper left: Irga6, upper right: LD540, lower left: phase contrast, lower right: merge. Scale bars represent 10 μM. b Quantification of 5a, showing percent of Irga6 structures co-localizing with LD540 (liquid droplets); 50 cells per sample were quantified and results of two independent experiments are shown. c gs3T3 cells stably transfected with inducible Irga6 were stimulated with mifepristone for 24 hours. Samples were fixed and stained as in 5a. Representative microscopic images of Irga6 and LD540 co-localization are shown. Arrows point at the Irga6 structures magnified at the end of each panel in the following array: upper left: Irga6, upper right: LD540, lower left: phase contrast, lower right: merge. d Quantification of 5c, showing percent of Irga6 structures co-localizing with lipid droplets; 50 cells per sample were quantified and results of two independent experiments are shown