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Fig. 3 | BMC Biology

Fig. 3

From: Surface attachment, promoted by the actomyosin system of Toxoplasma gondii is important for efficient gliding motility and invasion

Fig. 3

Loss of TgACT1 perturbs directed dense granule motions. a Fluorescence images of control LoxPAct1 parasites and LoxPAct1 parasites 72 h after rapamycin treatment. YFP (green); SAG1-∆GPI-mCherry to identify dense granules (yellow); anti-actin (pink). b Bar chart showing the percentage of granules exhibiting stationary (red), diffusive-like (blue) and directed (green) motion in control parasites and at 18, 36 and 72 h after rapamycin treatment. Hashed bars indicated the percentage of trajectories in each population when simulated pure 2D diffusion was analysed using the changepoint algorithm. c Parasite outline with dense granule trajectories overlaid from 10 control and 10 rapamycin treated parasites. Arrowhead indicates the parasites apical end

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