Fig. 5From: Surface attachment, promoted by the actomyosin system of Toxoplasma gondii is important for efficient gliding motility and invasionExpression levels of MyoC determine localisation to the inner membrane complex in the myoA knockout (KO) parasites. a Schematics of the plasmid (upper panel) and localisation of MyoC under different expression levels in both RH Δhxgprt and myoA KO (lower panel). a Constitutive overexpression (p5RT70-Ty-MyoC; left panel) and MyoC under its endogenous promoter (pMyoC-Ty-MyoC; middle panel), these constructs were randomly integrated. The endogenous MyoC locus was tagged in RH ΔKu80 via double homologous recombination (right panel). Schematic represents the final locus. Scale bar: 2 μm. b Corrected cell fluorescence intensities for different MyoC expression levels were measured using α-Ty. One-way ANOVA followed by Tukey’s post hoc test was used to compare means between groups. *** P < 0.0001, non-significance (ns) P > 0.05. c Plaque assay of myoA KO expressing the three different constructs. RH Δhxgprt was used as the control. The plaque area was measured using ImageJ with error bars representing ± standard deviation (n = 15 plaques). One-way ANOVA followed by Tukey’s post hoc test was used to compare means between groups. **** P < 0.0001, ** P < 0.001Back to article page