Fig. 8

Summary of vesicle capture by golgins. a Summary of the vesicle capture activities of the six indicated golgins. Golgin-97 and golgin-245 seem likely to capture the same type of vesicle, whilst the region of GCC88 that has capture activity has a very different sequence and therefore either captures the same vesicle by a different mechanism, or a different type of vesicle with overlapping cargo. The remaining three golgins capture intra-Golgi vesicles, and it appears that those bound to TMF have a similar but not identical set of cargo to those captured by golgin-84. There appear to be two different types of vesicle captured by GMAP-210. The precise number of classes of intra-Golgi vesicle, however, remains unclear. In all cases, the vesicle is captured by an N-terminal motif, with TMF also having a capture activity in its coiled-coil region. b Some of the possible models for how a vesicle captured at the N-terminus of a golgin could move closer to the Golgi membrane so as to allow vesicle fusion. The Rab binding sites on the golgin could be used in various ways to hold the golgin N-terminus closer to the membrane, or to induce a conformational change in the golgin. Alternatively, the vesicle could be captured by a further, more distant, golgin, or by different, shorter golgins (blue), to hold the vesicle closer to the target membrane. Of course, all these models are speculative, and other models could also apply