Fig. 5
From: Structural basis for potency differences between GDF8 and GDF11
Structural comparison of GDF8 and GDF11 in FS288-bound and apo-conformations reveal ligand flexibility. Cartoon diagrams of the bound and apo states of GDF8 (a) and GDF11 (b). Each monomer is colored independently to highlight the differences in conformation. Superposition of each conformation using monomerB (gray) is shown on the left. Protein Data Bank (PDB) IDs for bound ligands (FS288-bound GDF8 = 3HH2 [54]; antibody-bound GDF8 = 5F3B [61]; GDF11 = 5JHW) and apo ligands (GDF8 = 5JI1; GDF11 = 5E4G [46]; GDF11 = 5UHM). Resolution of apo-GDF8 was determined from recombinant human GDF8 produced and purified as previously described [54]. Resolution of FS288-bound and apo-GDF11 was determined from recombinant human GDF11 produced and purified by Acceleron Pharma