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Table 2 Antibodies for immunofluorescence staining

From: Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues

Label

Antibody

Clone

Company

Catalog number

RRID number

Alexa Fluor 488 conjugated

Anti-DARC

Clone 6B7

Generated in-house

  

anti-CD31

Clone 390 MEC13.3

Biolegend

102414

102502

AB_493408

AB_312913

anti-PNAd

Clone MECA-79

eBioscience

53-6036

AB_10804391

anti-MAdCAM-1

Clone MECA-367

Biolegend

120707

AB_493399

Anti-LYVE1

Clone ALY7

eBioscience

53-0443

AB_1633415

Rat IgG2a, κ

isotype control

Biolegend

400525

AB_389320

Rat IgM, κ

isotype control

Biolegend

400811

AB_1659271

PE conjugated

anti-CD31

Clone 390

Biolegend

102408

AB_312903

Anti-ICAM-1

3E2

BD Pharmingen

553253

AB_394735

Armenian Hamster IgG

Isotype control

Biolegend

400908

AB_326594

PE-Cy7 conjugated

anti-gp38

Clone 8.1.1

Biolegend

127411

AB_10613294

anti-TER-119

Clone TER-119

Biolegend

116222

AB_2281408

Alexa Fluor 647 conjugated

Anti-DARC

Clone 6B7

Generated in-house

  

rat IgG2a,k

isotype control

Biolegend

400526

AB_389342

APC-Cy7 conjugated

anti-TER-119

Clone TER-119

Biolegend

116223

AB_2137788

Anti-CD45.2

Clone 104

Biolegend

109824

AB_830789

anti-CD11b

Clone M1/70

Biolegend

101226

AB_10784810

Pacific blue conjugated

anti-CD45.2

Clone 104

Biolegend

109820

AB_492872

Anti-CD45

Clone 30-F11

Biolegend

103126

AB_493535

Anti-CD31

Clone 390

102422

AB_10612926

Brilliant violet 510 conjugated

Anti-CD45

Clone 30-F11

Biolegend

103138

AB_2563061

Alexa Fluor 546 conjugated

Anti-DARC

Clone 6B7

Generated in-house

  

Alexa Fluor 605 conjugated

Anti-CD31

Clone 390

Biolegend

102427

AB_2563982

Anti-CD45

Clone 30-F11

103139

AB_2562341