Fig. 1

Telomere shortening and metabolic characterization of G2-Terc^−/− mice. a Breeding scheme for second generation Terc KO mice. b Telomere length relative to Actb in male and female enterocytes (male n = 15 WT, n = 12 KO; female n = 8 WT, n = 14 KO, 14 months old). c Body weight, (d,e) Body composition and tissue weights at 14 months of age. male n = 18 WT, n = 15 KO (d), female n = 14 WT, n = 16 KO (e). f H&E stainings of perigonodal white adipose tissue (pgWAT), subcutaneous white adipose tissue (scWAT) and liver of male G2-Terc^−/− mice and controls and adipocyte cell size quantification in pgWAT and scWAT. Scale bar shows 100 µm. Adipocyte cell size quantification raw data are included in Additional file 3: Table S1a. g Serum triglyceride levels in 14 month old mice (male n = 17 WT, n = 14 KO; female n = 8 WT, n = 15 KO). h Fasting glycemia in 12 months old mice (male n = 12 WT, n = 14 KO; female n = 14 WT, n = 16 KO). i Percentage of glycosylated hemoglobin (HbA1c%) in 13 months old mice (male n = 18 WT, n = 15 KO; female n = 14 WT, n = 16 KO). j Fasting serum insulin levels in 12 month old mice (male n = 13 WT, n = 14 KO; female n = 14 WT, n = 16 KO). All data are shown as mean ± SE. Statistical analysis was performed using an unpaired t-test or ordinary two-way ANOVA with Sidak’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Red and black dots represent male mice. Green and purple dots represent female mice