Fig. 2

G2-Terc^−/− mice show improved insulin sensitivity and glucose tolerance. a Oral gavage glucose tolerance test (oGTT) in 12 months old male and female G2-Terc^−/− and control mice (male n = 12 WT, n = 14 KO; female n = 14 WT, n = 14 KO). b Intraperitoneal glucose tolerance test (ipGTT) in 12 months old male and female G2-Terc^−/− and control mice (male n = 12 WT, n = 14 KO; female n = 14 WT, n = 14 KO). c Insulin tolerance test (ITT) in 12 months male and female G2-Terc^−/− and control mice (male n = 12 WT, n = 12 KO; female n = 12 WT, n = 14 KO). d Pyruvate tolerance test (PTT) in 13 months male and female G2-Terc^−/− and control mice (male n = 12 WT, n = 11 KO; n = 14 WT, n = 13 KO). e,f Serum insulin (e) and glucose levels (f) during an oral glucose stimulated insulin secretion test (oGSIS) performed in 12 months old male and female G2-Terc^−/− and control mice (male n = 12 WT, n = 13 KO, female n = 14 WT, n = 14 KO). g Ex vivo glucose-stimulated insulin secretion test in isolated pancreatic islets from 14 month old male and female G2-Terc^−/− and control mice. Percentage of secreted insulin compared to total insulin content. Ten islets were used per well. Male:10 technical replicates from five WT mice and 11 technical replicates from five G2-Terc^−/− mice. Female: 12 technical replicates from four WT mice and 24 technical replicates from five G2-Terc^−/− mice. All data are shown as mean ± SE. Statistical analysis was performed using a two-way ANOVA followed by a Sidak’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Red and black dots represent male mice. Green and purple dots represent female mice