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Table 1 Parameters from a non-linear curve fit of ACh concentration–response curves in oocytes expressing Xenopus tropicalis α4β2 nAChRs

From: A potential cost of evolving epibatidine resistance in poison frogs

cRNA ratio

Receptor

EC50 (nM)

nH

Imax (%)

n

1:3

α4β2(FS)

47 (37 to 61)

0.85 ± 0.06

97 ± 2

18

α4β2(FC)

18 (13 to 26)

1.3 ± 0.2

92 ± 3

14

α4β2(LC)

21 (12 to 43)

2.2 ± 0.8

83 ± 4

6

7:1

α4β2(FS)

14 (8 to 30)

1.0 ± 0.2

90 ± 4

13

α4β2(FC)

20 (17 to 24)

1.5 ± 0.1

94 ± 1

10

α4β2(LC)

–

–

–

37

  1. The data from all curves fitted a one-population (monophasic) curve. β2(FS) represents F106 and S108 in the β2 subunit. When used for residues, the bold font indicates substitutions in the wild-type background
  2. Dashes signify currents too small to determine concentration–response curves
  3. EC50 effective concentration 50 (concentration that produces 50% of maximal response), expressed as mean (95% confidence intervals); nH Hill coefficient, expressed as mean ± SEM; Imax maximal current, expressed as a percentage of maximal response (mean ± SEM); n number of oocytes
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BMC Biology

ISSN: 1741-7007

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