Skip to main content

Table 3 Parameters from a non-linear curve fit of ACh concentration–response curves in oocytes expressing concatenated human α4β2 nAChRs (2 α4:3 β2)

From: A potential cost of evolving epibatidine resistance in poison frogs

Receptor

EC50 (µM)

nH

Imax (%)

n

α4β2 control

1.3 (1.2 to 1.5)

1.04 ± 0.05

96 ± 1

14

α4β2(FC) P3

1.4 (1.3 to 1.5)

1.06 ± 0.03

98 ± 1

14

α4β2(FC) P1, P3

–

–

0

7

α4β2(FC) P1, P3, P5

–

–

0

7

  1. The data from each curve fit a single population (monophasic) curve. β2(FC) represents F106 and C108 in the β2 subunit. The bold font indicates a substitution in the wild-type background. P1, P3, and P5 refer to the position of the β2 subunit in the concatemer (Fig. 4)
  2. Dashes signify no currents
  3. EC50 effective concentration 50 (concentration that produces 50% of maximal response), expressed as mean (95% confidence intervals); nH Hill coefficient, expressed as mean ± SEM; Imax maximal current, expressed as a percentage of maximal response (mean ± SEM); n number of oocytes
Back to article page

BMC Biology

ISSN: 1741-7007

Contact us