Fig. 2
From: A new compact adenine base editor generated through deletion of HNH and REC2 domain of SpCas9
Stacking domain deletions results in sABE. a Schematic of multiple domain deletions of ABE8.17. b Comparison of editing efficiencies of variants with multiple domain deletions in HEK293T cell line by plasmid transient transfection. c Representative fluorescence microscopy images of deletion variants analysis. d Comparison of editing efficiency of ABE8e deletion variants by flow cytometry analysis (FCA). e, f Efficient A-to-G conversions in 6 endogenous loci of sABE in HEK293T cells, and 7 endogenous loci in N2a cells. Error bars indicate SEM (n = 3)