Fig. 3
From: A new compact adenine base editor generated through deletion of HNH and REC2 domain of SpCas9
Characterization of sABE. a Comparison of editing efficiency in seven target sites using sABE and 8e-SaCas9-KKH (n = 3). b Summary of the A-to-G editing efficiency induced by sABE and 8e-SaCas9-KKH at the 7 target sites. c Summary of editing window for the sABE at endogenous genomic loci. Each data point represents the mean A-to-G editing efficiency at the indicated position of the spacer across 10 target sites, respectively (n = 3). d Comparison of the tolerance of sABE and ABE8e for mismatched sgRNAs, mismatched sgRNAs that differed from the site 8 by two nucleotides, mismatched nucleotides and the PAM sequence is shown in red and blue, respectively. Error bars indicate the SEM (n = 3). e DNA off-target analysis comparing sABE and ABE8e plasmid delivery, at VEGFA, HEK293-4, EMX1, HEK2, and TYRO3. Editing efficiencies and on-target: off-target editing ratios are shown