Fig. 4
From: A new compact adenine base editor generated through deletion of HNH and REC2 domain of SpCas9
Successful installation of mutations by sABE in cells and mouse embryos. a Schematic illustrates the design of sgRNAs for installation. sgRNAs with detectable editing in Sanger chromatogram are displayed. Editing efficiency was detected by Sanger sequencing and shown by Heatmaps. b Overview of tested sgRNAs at their respective target locus in murine Pcsk9 and correlated editing efficiency of splice-donor (SD) sites and splice-acceptor (SA) sites. c Target-site sequences within the targeted loci in mouse embryos, and the A-to-G editing frequencies at the target site using sABE. Target sequence (black), PAM region (red) and target sites (red)