Fig. 1

Introduction of DEE mutations using PE3, PE2max, and PE4max in HEK293T cells. White bars represent editing of the target of interest, grey bars represent presence of unintended SNVs/Indels at the edit locus, and black bars represent editing of the PAM site. A Validation of PE3 editing using pegRNAs targeting EMX1 (introduction of K263N and G265C) and SCN1A (introduction of R612*). B Editing efficiency using PE3 with a pegRNA that introduces the KCNQ2 R201H mutation together with an additional silent PAM-removing mutation following a single or double transfection. C Left: Sanger Sequencing results of R201H + PAM-removing mutation after two transfection rounds. Right: WES read count pie chart of R201H + PAM-removing mutation. D Comparison of PE3, PE2max, and PE4max editing efficiency using three different pegRNAs. E Comparison of G > C versus G > T conversion efficiency in SCN1A at position c.1863–1865, using PE2max and PE4max. The target region and designs of R612* and R201H + PAM pegRNAs can be found in Additional file 1: Fig. S9. Bar plots show the mean edit percentages ± SD; replicates are presented as individual data points (see individual data values in Additional file 2: Table S1)