Fig. 3
From: Increased prime edit rates in KCNQ2 and SCN1A via single nicking all-in-one plasmids
Correction of SCN1A R612* in HEK293TR612*/R612*. (Left) Comparison of the editing efficiency in correcting the SCN1A R612* mutation using dncRNA PE3 versus sncRNA pAIO-PE2-GFP and pAIO-PE4max. (Right) Sanger Sequencing results of HEK293Twt/wt, HEK293TR612*/R612* and treated HEK293T.R612*/R612* cells using pAIO-PE2-GFP. Black arrow indicated the SCN1A R612 location. The target region and design of R612* and *612R pegRNAs can be found in Additional file 1: Fig. S9. Bar plots show the mean edit percentages ± SD; replicates are presented as individual data points (see individual data values in Additional file 2: Table S1)