Fig. 4

Transcriptome analyses of the secretome of cultivar Zhongzhimian No.2 (ZZM2) in response to Verticillium dahliae. A Gene expression patterns of the secretome and their functional enrichment in response to V. dahliae infection. The resistant cultivar (cv. Zhongzhimian No.2, ZZM2) and susceptible cultivar (cv. Junmian No.1) with the time course (6, 12, 24, 48, 72 h post-inoculation) samples were performed for transcriptome analyses. The filtering parameters of DEGs were |log₂FoldChange|≥ 1.0 and Padj < 0.05. All differentially expressed genes (DEGs) were painted on the chromosomes according to their physical position. The DEGs of the same time point from the resistance cultivar (red columns) and susceptible cultivar (green columns) are shown as overlapping for comparison, and the columns showing “gene response” in both cultivars are shown in brown color. Secreted proteins classified in three KEGG annotation pathways (No. 2–4) and according to four gene ontology (GO) annotations (No. 5–8) are labeled in orange and blue lines, respectively. Pink triangles represent the candidates for functional validation, and the purple dots represent the selected candidates that were differentially expressed in response to V. dahliae. B Significant catalogs of Gene Ontology (GO) enrichment of DEGs in the ZZM2 secretome in response to the V. dahliae infection. The significant categories were selected by the Pearson chi-square test with P < 0.05, and the total secreted proteins of the secretome were set as the control. Information on GO categories is listed in Additional file 2: Table S8, and the GO category of hydrolase activity (GO:0016787) is labeled in bold font. C The expression pattern of phenylpropanoid biosynthesis-related genes in response to V. dahliae. The heatmap representation includes the five secretome members that are differentially expressed and function in the biological process of oxidation (K00430) and 29 members that are differentially expressed and function in the biological process of dehydrogenation (K22395). Letters “R” and “S” represent the resistant cultivar ZZM2 and the susceptible cultivar (cv. Junmian No.1), respectively. D Heat map analyses showing expression of the predicted secretome in response to V. dahliae in the resistant cultivar ZZM2 or the susceptible cultivar Junmian No.1. Blue boxes (left side) represent four Gene Ontology (GO) annotations