Fig. 3
From: Sensing and avoiding sick conspecifics requires Gαi2+ vomeronasal neurons
VSN Ca2+ responses to LPS-urine require Gαi2. a En face VNE confocal Ca2+ imaging approach. b High magnification image of Rhod2/AM loaded vomeronasal knobs (100 × 60 μm). c Mean knob density does not differ in cGαi2+/− vs. cGαi2−/− VNOs (n = 13, each; Mann-Whitney: ns, p = 0.61). d Images showing Rhod-2 fluorescence at rest (Fcontrol), at the peak of the response to male urine from PBS-treated mice (Fpeak PBS), and at the peak of the response to male urine from LPS-treated mice (Fpeak LPS). Bottom: ΔF images indicating responsive knobs to PBS- and/or LPS-urine (14 × 14 μm). e Example traces of confocal time-lapse recordings in single VSN dendritic knobs showing repeatable responses either to PBS-urine, to LPS-urine, or to both stimuli. f Density of knobs that responded to PBS-urine analyzed in control vs. cGαi2−/− mice (11 and 8 recording sites in 6 and 5 animals, respectively; Mann-Whitney, ***p < 0.001). g Density of knobs that responded to LPS-urine analyzed in control vs. cGαi2−/− mice (Mann-Whitney, *p < 0.05). h Density of knobs that reacted to both PBS- and LPS-urine analyzed in control vs. cGαi2−/− mice (unpaired t-test: t (17) = 0.29, p = 0.77). i Venn diagrams indicating the percentages of PBS- and LPS-urine responders and their overlap in control vs. cGαi2−/− VNEs (based on 440 and 272 responding cells, respectively)