Fig. 5

VSN Ca²⁺ responses to feces extract (FE) from LPS- and PBS-treated mice require Gαi2. a Example traces of confocal time-lapse recordings in single VSN dendritic knobs showing repeatable responses to PBS-feces, to LPS-feces, or to both stimuli. b–d Density of knobs responding exclusively to FE solution from either PBS- or LPS-treated mice analyzed in control vs. cGαi2^−/− VNEs. b Knobs that responded exclusively to PBS-feces in control vs. cGαi2^−/− VNEs (19 and 11 recording sites in 10 and 5 animals, respectively; Mann-Whitney ns, p = 0.25). c Knobs that responded exclusively to LPS-feces (Mann-Whitney, ***p < 0.001). d Knobs that responded to both stimuli (Mann-Whitney, **p < 0.01). e Density of knobs that responded to PBS- and LPS-feces, either selectively or together (n = 19 and 11, respectively; Kruskal–Wallis ANOVA, p < 0.001; Mann-Whitney, *p < 0.05; ** p < 0.01; ***p < 0.001). f Venn diagram of response percentages to PBS- and LPS-feces in control knobs (based on 444 responding knobs)