Fig. 2

Timelapse quantification of CMTs reorganization on 4-day-old hypocotyls in response to tensile stress. A–L Confocal images of microtubule reporter lines, GFP-MBD (A, E, I), bot1-7 GFP-MBD (B, F, J), mCit-MBD (C, G, K), and GFP-TUA6 (D, H, L) shown at three time points of the time series, 0, 120 and 240 min after the ablation (see full timelapse in Supplemental Fig. S6, S9, S12, and S15). The red lines represent the relative anisotropy by their length and the main orientation of the CMT arrays in the corresponding cells (scale bars are 50 µm). M–T Plots of the mean and bootstrapped 95% confidence interval for angle to ablation (M–P) and anisotropy (Q–T) for GFP-MBD (ablation: n = 81 cells in 9 hypocotyls; no ablation: n = 67 cells in 9 hypocotyls; M and Q), mCit-MBD (ablation: n = 75 cells in 9 hypocotyls; no ablation: n = 69 cells in 9 hypocotyls; N and R), GFP-TUA6 (ablation: n = 86 cells in 9 hypocotyls; no ablation: n = 73 cells in 9 hypocotyls; O and S) and bot1-7 GFP-MBD (ablation: n = 71 cells in 9 hypocotyls; no ablation: n = 66 cells in 9 hypocotyls; P and T). The blue lines correspond to the samples with ablation and the orange lines, mock samples without ablation. The raw values used to generate this graph and the summary statistics plotted in the graphs can be found in Additional file 4: Supplemental Table S1, S2, and S3. All the data represented in this figure is also represented as average per samples as well as individual cells in Additional file 3: Supplemental Fig. S7, S8, S10, S11, S13, S14, S16, and S17