Fig. 2

Residues T323/M324/R325 are crucial for synaptic vesicle exocytosis. A Sample traces (left), summary graphs of frequency (middle), and amplitude (right) of mIPSC. Data were recorded from cultured cortical neurons infected with control lentivirus (control), lentivirus expressing Munc18-1 shRNA (none), and rescued expressing sequences of Munc18-1 WT (WT) or Munc18-1 TMR mutant (TMR). B Sample traces (left), summary graphs of amplitude (middle), and charge transfer (right) of evoked IPSCs triggered by action potential recorded from cultured cortical neurons as described in A. C Sample traces (left) and summary graphs of charge transfer (right) of hypertonic sucrose-evoked IPSC recorded from cultured cortical neurons as described in A. Data are presented as mean ± SEM. Statistical significance was analyzed by Student’s t test; ^**, P < 0.01; ^***, P < 0.001. Cells recorded are from at least three independent cultures, and the cell numbers are shown in bars. KD, knockdown