Fig. 3
From: Identification of residues critical for the extension of Munc18-1 domain 3a
The TMR mutation supports Syx1 transport. A Representative images of HEK293T cells transfected with plasmids expressing EGFP-Munc18-1 and mCherry2-Syx1 respectively. B Representative images of HEK293T cells cotransfected with mCherry2-Syx1 and EGFP-Munc18-1 WT (WT) or TMR mutant (TMR). C Quantification of Munc18-1 and Syx1 distribution on the plasma membrane. Pictures used for analysis are from three independent cultures, and the numbers are listed in the bars. Data are shown as mean values ± SEM. Statistical significance was analyzed by Student’s t test. ***, P < 0.001. Scale bar: 20 μm; PM: plasma membrane