Fig. 3
Evaluation of PACSI on HNSC data. A The UMAP visualization of the HNSC scRNA-seq dataset. B The UMAP visualization of PACSI-identified cells. The red dots are PACSI-identified cells associated with HNSC. C The distribution of PACSI-identified cells by cell types. D Rank for regulons in PACSI-identified cells associated with HNSC based on RSS. E Differential gene expression analysis. The x-axis shows the difference in the percentage of cells expressing the gene between PACSI-identified cells and the others; the y-axis represents the log2 fold-change. F The significantly enriched Hallmark pathways in the PACSI-identified cells compared to other cells using GSEA. G Box plot shows the enrichment scores of the HNSC signature in the HNSC and normal samples from the independent validation dataset. A two-sided Wilcoxon rank-sum test was performed to estimate the significance level