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Fig. 1 | BMC Biology

Fig. 1

From: An automated, low-latency environment for studying the neural basis of behavior in freely moving rats

Fig. 1

Workflow for the design, preparation, execution, and initial analysis of behavioral experiments as they pertain to the RIFF. The design of an auditory-guided task includes the development of task logic, the nature of rewards and punishments, as well as the selection of the auditory stimuli. The flexibility of the RIFF allows for substantial freedom in these choices. Rat preparation includes habituation to the RIFF and electrode implantation. While running the experiment, the RIFF is designed to keep a tight synchronization of all real-time events, and to ensure efficient data storage. The stored data allows for offline analysis that includes spike sorting and animal pose estimation, which are then precisely aligned with the rest of the information from the experiment

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