Fig. 1

Distribution of DHSs in the cotton genome and identification of teDHSs. A Distribution of DHSs in the cotton genome. The x-axis shows the percentage of DHSs associated with each type of genomic location. B UpSet plot of teDHSs during fiber development. The left bars show the number of total teDHSs at different periods. The right bar plot and points represent the number of teDHSs in specific intersection. C Motifs were significantly enriched in teDHS. We calculated the ratio of means of significant enrichment motifs during fiber development in teDHSs to those in non-teDHSs. D Fluorescent, bright-field and merge (bright-field and red fluorescent) confocal laser scanning micrographs of cotton leaf cells bombarded with #TE1-mini35s:RFP construct DNA-loaded gold particles are presented. 35S:RFP is a positive control with a construct with RFP expression driven by the 35 s promoter. mini 35 s:RFP is a negative control with a construct with RFP expression driven by the mini 35 s promoter. #TE1-mini35s:RFP is a construct of validation of enhancer function, which conducted using a construct with RFP expression driven by the mini 35 s promoter and teDHSs. RFP expression in the constructs with teDHSs indicates that the teDHSs promote gene expression. Scale bar, 25 µm