Fig. 6

Roles of TFs in fiber development revealed through regulatory networks. A GhNAC2 (GH_D06G0471) interact with GhWRKY46 (GH_A06G2073). The DNase-seq tracks for the GhNAC2 loci are shown. The WRKY46 recognition motif “CNTTGAC” (large coded) was identified from the upstream DHSs of GhNAC2. Orange is DAP-seq and peaks. Shade boxes indicates DHS region. B The phenotypes of plants inoculated with pTRV:00 and pTRV:GhWRKY46. The pTRV:00 vector was used as a vector control. C Relative transcript levels of GhWRKY46 in systemic leaves of cotton infiltrated with pTRV:00 and pTRV:GhWRKY46. The WT value was set as 1. Data are shown as mean values ± SD (n = 3). (Student’s t test, *** p < 0.001). D Cross-cut lignin histochemical staining analysis of cotton stalks. I: Primary xylem; II: Primary phloem. Scale bar, 100 µm. E Primary xylem area proportion in systemic stem of cotton infiltrated with pTRV:00 and pTRV:GhWRKY46. (Student’s t test, * p < 0.05, ** p < 0.01, N.S, not significant, n = 12, 10, 9, respectively)