Fig. 1
From: A stromal lineage maintains crypt structure and villus homeostasis in the intestinal stem cell niche
Lineage tracing and scRNA-seq analysis of Twist2 lineage cells in the intestinal villi. a Detection of stromal lineages labeled by Twist2, Prrx1, or Nestin, and Acta2+ myofibroblasts in the small intestinal villi of adult mice. Right panel: the percentage of Twist2, Prrx1, and Nestin lineage cells and Acta2 lineage myofibroblasts in non-epithelial (EpCAM+) cells. Bar: 50 μm. The calculation was done by counting Tomato+ cells on six views per mouse and divided by the number of non-epithelial DAPI+ nuclei, N = 6. b Single-cell transcriptome analysis of small intestinal cells. A scRNA-seq dataset (PMID 32084389) was used to analyze the expression of various stromal/myoblast cell markers