Fig. 4
From: A stromal lineage maintains crypt structure and villus homeostasis in the intestinal stem cell niche
Twist2 cell-secreted Wnts were critical for villus and crypt development. a Detection of Twist2 lineage cells in E14.5, E16.5, and p12 Twist2-Cre;Tomato mouse intestines. Bar: 50 μm. b Quantitative PCR results of the intestinal stromal cells (Tomato+) of E16.5 control, Twist2-Cre;Wlsf/f, and Twist2-Cre;Wls+/f embryos. N = 4. c Representative immunohistochemical staining results for β-Catenin in the intestinal villi of E16.5 control, Twist2-Cre;Wlsf/f, and Twist2-Cre;Wls+/f embryos. Bar: 50 μm. Brown color indicated β-Catenin signals. d H/E, Ki67, and goblet staining of intestinal villi of E16.5 control, Twist2-Cre;Wlsf/f, and Twist2-Cre;Wls+/f embryos. Bar: 50 μm. Bottom panels: quantitation data. ***P < 0.001. N = 8 or N = 7 (Ki67). e Quantitative PCR results of the intestinal stromal cells (Tomato+) of P12 control and Twist2-Cre;Wls+/f pups. N = 4. Brown color indicated β-Catenin signals. f Representative immunohistochemical staining results for β-Catenin in the intestinal villi of P12 control and Twist2-Cre;Wls+/f pups. Bar: 50 μm. g H/E, Ki67, goblet, and Paneth cell staining of intestinal villi of p12 control and Twist2-Cre; Wls.f/f embryos. Bar: 50 μm. Bottom panels: quantitation data. *P < 0.1, **P < 0.01, and ***P < 0.001. N = 7