Fig. 6
From: A stromal lineage maintains crypt structure and villus homeostasis in the intestinal stem cell niche
Crypt regeneration defects in Twist2-Cre; Wls+/f mice were rescued by antibiotics treatment. a Decreases in the number of Paneth cells and expression of AMPs in regenerating villi of Twist2-Cre; Wls+/f mice. Bar: 50 μm. b qPCR analysis showed reduced expression of Crypt4 in the villi of villi of Twist2-Cre;Wls+/f mice. The basal levels in Twist2-Cre; Wls+/f mouse intestines were set at 1.0. ***P < 0.001. N = 4. c IR-induced production of inflammatory cytokines in the intestine was enhanced in Twist2-Cre; Wls+/f mice, which was rescued by antibiotic treatment. The basal levels of cytokines in normal mouse intestines were set at 1.0. *P < 0.05, **P < 0.01, and ***P < 0.001. N = 3. d Crypt regeneration defects in Twist2-Cre; Wls.+/f mice were rescued by antibiotics treatment. Bar: 50 μm. Bottom panels: quantitation data. *P < 0.05, **P < 0.01, and ***P < 0.001. N = 5