FACT regulates pluripotency through proximal and distal regulation of gene expression in murine embryonic stem cells

Table 2 Overlap between SPT16-V5 CUT&RUN peaks and OCT4, SOX2, and NANOG ChIP-seq peaks

Peak overlap category	OCT4 peaks	Sox2 Peaks	Nanog Peaks	Any of OSN
V5 Peaks	8544	5948	5307	9682
OSN Peaks	45,476	19,211	16,817	52,899
% of OSN peaks bound by FACT	18.33%	30.72%	31.40%	17.91%
% of V5 peaks bound by OSN	52.41%	36.85%	32.94%	59.63%
V5-bound promoters	4261	2719	2327	4452
OSN-bound promoters	6550	1542	666	6948
V5- and OSN-bound promoters	2040	801	343	2202
OSN-bound TSS-distal peaks	38,926	17,669	16,151	45,938
V5-bound TSS-distal OSN peaks	6504	5147	4964	7480

To be called as overlapping, peaks must have directly shared at least 1 bp and passed peak-calling thresholds (see “Methods”)

ISSN: 1741-7007