Fig. 5

GWAS identification of candidate genes associate with stem length. a Manhattan plots for stem length in 241 accessions using MLMM. The black dashed lines indicate the significance threshold (p value = 2.0 × 10^–5) and black arrow indicates the significant GWAS peak. b Box plot of stem length, the white box and gray box represent Hap.G and Hap.K, respectively. c Relative expression of LjZCB in different accessions carrying Hap.G and Hap.K, respectively. The white bar and gray bar represent Hap.G and Hap.K, respectively. d Subcellular localization of LjZCB protein in Arabidopsis. LjZCB-GFP constructs were co-transformed with AtH2B-Mcherry into Arabidopsis mesophyll protoplasts and examined by confocal laser scanning microscopy. Confocal micrographs are shown brightfield, chloroplast auto-fluorescence, green fluorescent protein (GFP), and the merged images from left to right. AtH2B-Mcherry was used as nuclear localization maker. Bar, 5 μm. e The box plot of Arabidopsis root length in WT e and 3 lines of OE-35S::LjZCB 7 days after germination. f The box plot of Arabidopsis stem length in WT and 3 lines of OE-35S::LjZCB 50 days after germination. g Image of root length of WT and OE-35S::LjZCB line 3 Arabidopsis 7 days after germination. White line represent bar, 2 cm. h Image of stem length of WT and OE-35S::LjZCB line 3 Arabidopsis 50 days after germination. White line represent bar, 2 cm. The significance of difference was derived with two-tailed t-test (*P < 0.05, **P < 0.01, and ***P < 0.001)