Fig. 6
GWAS identification of candidate genes associate with plant height. a Manhattan plots for plant height in 241 accessions using MLMM. The black dashed lines indicate the significance threshold (p value = 2.0 × 10.−5) and black arrow indicates the significant GWAS peak. b Gene model of Lj3g3v0075610. Exons, introns, and promoter are represented by blue boxes, black lines, and red line, respectively. The SNP in promoter is marked by a black arrow. c Box plot of stem length, the white box and gray box represent Hap.C and Hap.Y, respectively. The significance of difference was derived with two-tailed t-test (*P < 0.05, **P < 0.01, ***P < 0.001). d Relative expression of Lj3g3v0075610 in different accessions carrying Hap.C and Hap.Y, respectively. The white bar and gray bar represent Hap.C and Hap.Y, respectively. e Schematic diagram of reporter and effector in Arabidopsis protoplast transactivation assays. The LjZCA promoter (pZCA) and mutated pZCA-D87 fused with firefly luciferase gene (LUC), respectively. NOS, the transcriptional terminator of the nopaline synthase gene from Agrobacterium tumefaciens. f Luciferase assays of pZCA, and pZCA-D87. Values represent the mean ± SE of triplicate experiments. Significant differences between values are indicated with different letters (*, P < 0.05 one-way ANOVA)