Fig. 3

The activation of excitatory neurons was associated with WMC. a Representative images showing the FOS⁺ neurons, CaMKII⁺ neurons and co-localized cells in the PrL of each group of mice. b Representative images showing the FOS⁺ neurons, GAD⁺ neurons and co-localized cells in the PrL of each group of mice. c The number of FOS⁺ neurons co-localized with CaMKII⁺ neurons (Two-way ANOVA, interaction: p > 0.05, task: p < 0.0001, genotype: p < 0.05; post hoc Tukey’s tests, *p < 0.05, ****p < 0.0001). No task: n = 15 slices from five mice per group; Task: n = 12 slices from four mice per group. d Relationship between the amount of FOS⁺ neurons and FOS⁺ neurons co-localized with CaMKII⁺ neurons (Linear regression, ****p < 0.0001). n = 54 slices from eighteen mice. e The number of FOS⁺ neurons co-localized with GAD⁺ neurons (Two-way ANOVA, all p > 0.05; post hoc Tukey’s tests, all p > 0.05). n = 9 slices from three mice per group. Data are presented as the mean ± SEM