Fig. 4

Integration of genome accessibility and gene expression data. A Genomic distribution of ATAC-seq peaks identified at E7.5 (red), E8.5 (blue) and distribution of only the differentially accessible peaks (black). B Volcano plot of ATAC-seq peaks (|Log₂(Fold Change)|≥ 2 & p-value < 0.05). Significantly open regions at E8.5 in red, closed regions at E8.5 in green and non-significant in black. C Scatterplot showing correlation between genomic accessibility and gene expression. Significantly accessible and upregulated genes in red, closed and downregulated genes in green. D Top 3 GO biological process terms of positively regulated genes at E8.5 (red group in C), Abbreviations: Emb, Embryonic; Sys, System, A/P, Anterior/Posterior. E Top 3 GO biological process terms of negatively regulated genes at E8.5 (green group in C) (F) Heatmap of Log₂(Fold Change) of ATAC-seq and RNA-seq signals