Fig. 6
From: Regulatory changes associated with the head to trunk developmental transition
Characterization of Wnt5a enhancer, CR1. A ATAC-seq tracks showing accessibility profiles in the CR1 region. Phylogenetic conservation data (phyloP) [80] is shown in dark blue. B β-gal staining of CR1-β-gal transgenic embryo (n = 3/10), also showing dorsal view of the caudal region. C-F Whole-mount in situ hybridization of wild type and Wnt5a∆CR1/∆CR1 embryos at E9.5 and E10.5 using a probe for Wnt5a. (C’ and D’) Dorsal view of the posterior end of the embryo emphasizing the reduction of Wnt5a expression in the neural tube (brackets). At E10.5 Wnt5a expression is reduced in the PSM (arrows in E’ and F’). (G) RT-qPCR analysis of Wnt5a gene expression in wild type and Wnt5a∆CR1/∆.CR1 embryos at E9.5 and E10.5. Wnt5a expression is normalized to β-Actin. Three individual embryos were used for each condition. Values provided in Additional file 1: Table S4. Error bars indicate the standard deviation; **, p-value < 0.01 and *, p-value < 0.05