Table 1 gRNA and ssDNA used for CRISPR/Cas9
From: Regulatory changes associated with the head to trunk developmental transition
Raldh2 | Raldh-gRNA-up | AGGGAATGGCAGAACTCAGAGAGT |
Raldh-gRNA-down | AAACACTCTCTGAGTTCTGCCATT | |
Raldh2-3X-Stop | CATATCCCATTTTCTTGTGTCCCTTCTGTAGATCTTTATTAACAATGA ATGGCAGAACTCAGAGTGATAGAATTCAGTGGGAGAGTGTTCCCTG TCTGTAATCCAGCCACAGGAGAGCAAGTGTGTGAAGTTCAAGAAG | |
CR1 | gRNA_CR1_1 | CCAGTGGCAGTATTCTGTGA |
gRNA_CR1_2 | CTGTGTAGCCGTAGTTTGCC | |
ssDNA | CCCCCTAACCTCAAGGGAGCCTTTGTCCCCCACAGGCTAGTGG CCAGTGGCAGTATTCTGGCCAGGAGGTGAGGGACTTCCACAA ACTGGAGGCTCTCCTTTGGGAGTCTTCCCCAGTGG | |
CR2 | gRNA_CR2_1 | TCTTTCGGTCGTTCCCAGAG |
gRNA_CR2_2 | GATACAACCGTCTTCTAGCT | |
ssDNA | CCATCGGGGCGGGTGAGACCTCTCAGCACACCCTCTGTCCCCT TCTTTCGGTCGTTCCCAGCTAGGGAACCAGGGCAAAGTTGGC CTGGGTGGGATGGTTCTAAGGGTGCAGGGTGAACA | |
CR2a | gRNA_CR2a_1 | ATTGGAGGTGCACTGGGTGA |
gRNA_CR2_2 | GATACAACCGTCTTCTAGCT | |
ssDNA | GGATGCTGGTGTGTATGCTTGTATGTGCCTTTGGAGTCAGGGT ATTGGAGGTGCACTGGGGCTAGGGAACCAGGGCAAAGTTGG CCTGGGTGGGATGGTTCTAAGGGTGCAGGGTGAACA | |
CR2b | gRNA_CR2_1 | TCTTTCGGTCGTTCCCAGAG |
gRNA_CR2b_2 | TCTCCTGGGCATTATCTGCC | |
ssDNA | CCATCGGGGCGGGTGAGACCTCTCAGCACACCCTCTGTCCCCT TCTTTCGGTCGTTCCCAGCCAGGTTCACCCCATTTCTTTTTATAA TCTTACTACATATTTAAAGGAGTCCCTTGCCT |