Fig. 2

FAAP100 is essential for PGC development. A Representative images of E15.5 and E13.5 gonads from wild-type and Faap100^−/− embryos co-immunostained for DDX4 (germ cell marker) and FOXL2 (pre-granulosa marker, in the ovaries) or SOX9 (Sertoli cell marker, in the testis). Scale bars, 50 μm. B Representative alkaline phosphatase staining images of wild-type and Faap100^−/− embryos at E8.5 and E9.5 or genital ridges at E11.5. Scale bars, 200 μm. C Quantification of PGCs (AP⁺ or STELLA⁺) in wild-type and Faap100^−/− embryos at various embryonic stages as indicated. n = 12/10/6/6/7/7 embryos. Data are shown as the mean ± SD (C). Unpaired two-tailed Student’s t-test (C), ns, not significant, *P < 0.05 and ***P < 0.001