Fig. 4

Assessing effects of paternal Cort-treatment on offspring urinary MUP levels and liver gene expression. A–B urinary creatinine or total MUP were not affected by paternal Cort-treatment. n = 16/13. C after SDS-PAGE of mouse urine, three different MUP bands can be seen, whose molecular weight correspond to previously published literature. Heavier band = ‘Small MUP’, middle band = ‘Big MUP’, lighter band = ‘Darcin’. D–F paternal Cort-treatment induces reduced specific MUP populations in the urine. n = 16/13. G time being investigated by the female does not correlate with urinary Darcin concentration. n = 16/13. H–K Mup gene expression in the liver was not affected by paternal Cort-treatment. n = 16 per group. L–M Mup genes correlate with specific MUP populations in PatCort, but not in PatCT. n = 16/13. A–B unpaired t-test with Welch correction. D and E unpaired t-test with Welch correction. F Mann–Whitney test. G correlation of Pearson and simple linear regression. H: unpaired t-test with Welch correction. I: Mann–Whitney test. J and K: unpaired t-test. L–M: correlation of Pearson and simple linear regression. Data represented as mean ± standard deviation. * p < 0.05