Fig. 1

4-parameter analysis of modified Rotarod test of 48 mice on the fourth day of training. 4 trials per mouse and in total 192 trials were involved (7 trials went through testing but without recording due to technical problems). A Scheme illustrating time (s) on the x-axis and speed (rpm) on the y-axis in the accelerated Rotarod test. The test started with an acceleration phase from 4 to 20 rpm within the first 120 s, followed by constant speed for the subsequent 3 min. Maximal distance achieved at a given time is dependent on the rotation speed, as demonstrated by the polygons. Although three polygons with dashed lines and grey background had the same duration, polygon 3 exhibited the greatest distance due to higher speed. B The sequence of the run with longest duration during 5 min trial. Each run ends with a fall, each trial lasts 5 min. Ideally, mice exhibiting excellent performance, without any falls during the entire 5 min test have only one run, and in these cases, the run with the longest duration was considered as 1^st run. However, longest duration in 42 out of 185 trials was not in the first run. C The sequence of the run with maximal distance within each 5 min trial. Maximal distance in 93 out of 185 trials is not in the first run. D-G 4-parameter analysis of individual trials (Trial 1 to 4): First Latency, Longest duration, Maximal distance and Number of falls. The statistical evaluation was carried out by the Friedman test (matched data, non-parametric) followed by Dunn’s correction. n = 48 animals, asterisks indicate significances: **p < 0.01, ***p < 0.001, ****p < 0.0001