Fig. 8
From: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
Localization of AmyE in B. subtilis and B. licheniformis cells determined by its activity. A Cells were mounted on agarose slides for immobilization of cells and incubated for 30 and 60 min, respectively, at 37 °C with starch-BODIPY-FL. Fluorescence is produced by hydrolysis of this substrate when AmyE is secreted to the outer level of the cell wall. B Cells from three independent experiments displaying fluorescent signal were counted and normalized to the number of all analyzed cells