Fig. 9
From: Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
Single molecule tracking of SecA and SecDF. A Jump distance analyses of SecA-mNeonGreen (SecA-mNG) according to SQD analyses. The left panels represent wild type cells, right panels describe cells carrying the AmyE expression plasmid. The solid lines represent slow diffusing/static molecules, dotted lines are fits for medium-mobile molecules, dashed lines represent the fast-mobile population. B same as A for SecDF-mNeonGreen (mNG). C Bubble plots showing results from fitting of three populations by SQD analysis of single molecule tracks for SecA-mNeonGreen, size of bubbles corresponds to population size, diffusion constants are given on the y-axis, and bars in bubbles represent fitting errors. D bubble plot for SecDF-mNeonGreen data. E Heat map of static tracks of SecA-mNeonGreen projected into a 3 × 1 µm large cell, F similar as E for SecDF-mNeonGreen. F Plot of the probability density function of events of molecules staying within a radius of 106 nm for a certain amount of time (shown on the X-axis). The exponential decay curve can be explained by assuming two populations, one with a shorter (green curve) and one with a longer average dwell time (red curve), as stated in the inset