Beyond rational—biosensor-guided isolation of 100 independently evolved bacterial strain variants and comparative analysis of their genomes

Table 1 Most significant hotspot genes identified in the genomes of 100 independently generated C. glutamicum CgHis2 variants accumulating increased l-histidine concentrations

#	Locus tag	Gene	Enzyme	Nonsynonymous mutations in x variants
1	NCgl2409	fasB	Fatty acid synthase	24
2	NCgl2964		DEAD/DEAH box helicase	23
3	NCgl0802	fasA	Fatty acid synthase	22
4	NCgl2773	pks	Type 1 polyketide synthase	21
5	NCgl0184	emb	Arabinosyl transferase C	20
6	NCgl0181	gltB	Alpha subunit of glutamate synthase	19
7	NCgl0552		DNA segregation ATPase	19
8	NCgl2618	cps	Non-ribosomal peptide synthase	18
9	NCgl0040	pknB	Serine/threonine protein kinase	17
10	NCgl2959		Phosphoesterase	17
11	NCgl1737		Hypothetical membrane protein	17
12	NCgl2433	dinG	Probable ATP-dependent DNA helicase-related protein	15
13	NCgl2981		Hypothetical protein	15
14	NCgl0916	ggtB	Gamma-glutamyltranspeptidase	14
15	NCgl0705		Probable ATP-dependent helicase	13

ISSN: 1741-7007