Beyond rational—biosensor-guided isolation of 100 independently evolved bacterial strain variants and comparative analysis of their genomes

Table 2 Constructed C. glutamicum CgHis2 strains with individual gene deletions and the respective l-histidine titer obtained in microtiter plate-scale cultivations

C. glutamicum strain	Locus tag	Protein/enzyme	His-Titer [mM]	His-Titer [%]	Biomass [final OD₆₀₀]
CgHis2 reference			11,5 ± 0.6	-	19.3 ± 0.5
CgHis2 ΔfasB	NCgl2409	Fatty acid synthase	14.3 ± 0.4	+ 25	18.4 ± 0.7
CgHis2 ΔNCgl2964	NCgl2964	DEAD/DEAH box helicase	10.6 ± 0.3	− 8	18.5 ± 0.2
CgHis2 ΔfasA	NCgl0802	Fatty acid synthase	No deletion possible
CgHis2 Δpks	NCgl2773	Type 1 polyketide synthase	No deletion possible
CgHis2 Δemb	NCgl0184	Arabinosyl transferase C	No transformation of pSenHis[hisEG] possible
CgHis2 ΔgltB	NCgl0181	Alpha subunit of glutamate synthase	11.6 ± 0.2	0	21.9 ± 0.1
CgHis2 ΔNCgl0552	NCgl0552	DNA segregation ATPase	11.1 ± 0.6	− 4	19.6 ± 0.6
CgHis2 Δcps	NCgl2618	Non-ribosomal peptide synthase	13.6 ± 0.2	+ 18	18.6 ± 2.3
CgHis2 ΔpknB	NCgl0040	Eukaryotic-type serine/threonine kinase	No deletion possible
CgHis2 ΔNCgl2959	NCgl2959	Phosphoesterase	10.9 ± 0.2	− 6	19.4 ± 0.4
CgHis2 ΔNCgl1737	NCgl1737	Hypothetical membrane protein	10.8 ± 0.3	− 8	18.8 ± 0.2
CgHis2 ΔNCgl2981	NCgl2981	Hypothetical protein	No deletion possible
CgHis2 ΔggtB	NCgl0916	Putative gamma-glutamyltranspeptidase precursor PR	10.3 ± 0.4	− 12	20.0 ± 0.4
CgHis2 ΔNCgl0705	NCgl0705	Probable ATP-dependent helicase	11.8 ± 0.5	+ 2	18.8 ± 0.5
CgHis2 ΔputA	NCgl0098	Proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase	9.7 ± 0.4	− 17	20.3 ± 0.5
CgHis2 Δpyk2	NCgl2809	Pyruvate kinase	10.6 ± 0.3	− 8	18.1 ± 0.4
CgHis2 Δpyk1	NCgl2008	Pyruvate kinase	12.1 ± 0.4	+ 5	21.5 ± 1.0

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