Fig. 3
The late-occurring I1532T mutation has a stronger selective advantage than the F1534S mutation. A Structural and functional changes in VGSC caused by I1532T. Final stable structure at 200 ns simulation time of VGSC_WT superposed with VGSC_I1532T. VGSC_WT is colored in green and VGSC_I1532T in yellow. The residue Ile1532 and mutated Thr1532 are shown in stick view with corresponding color. B System flexibility analysis of VGSC_WT and VGSC_I1532T. The root-means-square deviation (RMSD) of the backbone of VGSC_WT is less than 8.0 angstrom and that of VGSC_I1532T is less than 7.0 angstrom, and the system achieves equilibrium within the simulation time. C The docking scores of deltamethrin molecules with VGSC_WT [14], VGSC_F1534S [14] and VGSC_ I1532T proteins. D The binding model of deltamethrin on the molecular surface of VGSC_I1532T. Deltamethrin is colored in cyan, and the molecular surface of protein is colored in pale yellow. E The 3D binding mode of deltamethrin interaction with VGSC_I1532T. Deltamethrin is colored in cyan, the surrounding residues in the binding pockets are colored in yellow, and the backbone of the receptor is depicted as a white cartoon with transparency. F Male mating competitiveness in different strains. Genotypes of offspring from female adults of different strains after successful mating with male adults from different strains. G Mating success rate between R30-1532 T and R30-1534S (n = 3). ***P < 0.001. H Genotypes of adults on the first and second day of emergence in Lab-R30 (n = 3). Homozygous wild-type is represented as SS, heterozygous as RS and homozygous mutant as RR. SS/SS: 1532I/I1534F/F, RS/RS: 1532I/T1534F/S, RR/SS: 1532 T/T1534F/F. I I1532T and F1534S mutations in vgsc gene in Lab-R strain were located on different chromosomes of a homologous pair. J The percentage of starved females from each strain successfully taking a human blood meal (n = 4). K Mean time for starved females from each strain to locate a host (n = 4). Different lowercase letters, a and b, represent significant differences (P < 0.05). The results in G, H, J and K are represented as the mean ± SEM